Constructing Lambda Red-Swaps in Salmonella
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چکیده
Homologous recombination is very efficient in many organisms. For example, short PCR products have been used extensively to generate gene replacements in the yeast Saccharomyces cerevisiae (reviewed in [Que and Winzeler 2002; http://www.openbiosystems.com/productPage.php?pageType=yeast.knockout&q=0]). Very short regions of homology (from 25 to 50 bp) are sufficient for efficient recombination in this system, with nearly 95% of the transformed cells carrying the desired gene replacement. In contrast, short linear DNA fragments are poor substrates for recombination in many bacteria.
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تاریخ انتشار 2004